Controlling vegetative cells and spores growth of Bacillus spp. using ethanolic Ketapang (Terminalia catappa L.) leaf extract

Terminalia catappa L. is a large, spreading type of tree which usually grows in tropical environment, especially at coastal area with sandy stones. The current study evaluated anti-Bacillus potential the ethanolic ketapang (Terminalia L.) leaf extract (EKLE) as antibacterial and sporicidal agent against vegetative cells spores Bacillus spp. activity EKLE (B. cereus ATCC33019, B. pumilus ATCC14884, subtilis ATCC6633 megaterium ATCC14581) were determined by performing well diffusion assay (WDA), minimum inhibition concentration (MIC), bacterial (MBC) time-kill curve analyses. was tested different concentrations EKLE. Then, extract's stability terms activities upon exposure to temperatures pHs carried out. Results demonstrated zones range 9.25 ± 0.75 mm - 11.67 0.47 mm. All inhibited MIC values 0.63–1.25 mg/mL can be completely killed MBC 0.63 >5.00 mg/mL. Time-kill analysis showed all 2.50–5.00 from 1 4 h. 1% (w/v) time. not affected (4, 30, 50, 80 121 °C) (3, 7 10), revealing conditions. In conclusion, exhibits spp., therefore, developed agent, paving way for its utilization food industry natural preservative.